|Other Names||ER lumen protein-retaining receptor 3, KDEL endoplasmic reticulum protein retention receptor 3, KDEL receptor 3, KDELR3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13180a was selected from the N-term region of KDELR3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L (By similarity).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Retention of resident soluble proteins in the lumen ofthe endoplasmic reticulum (ER) is achieved in both yeast and animalcells by their continual retrieval from the cis-Golgi, or apre-Golgi compartment. Sorting of these proteins is dependent on aC-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) inanimal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. Thisprocess is mediated by a receptor that recognizes, and binds thetetrapeptide-containing protein, and returns it to the ER. Inyeast, the sorting receptor encoded by a single gene, ERD2, is aseven-transmembrane protein. Unlike yeast, several human homologsof the ERD2 gene, constituting the KDEL receptor gene family, havebeen described. KDELR3 was the third member of the family to beidentified, and it encodes a protein highly homologous to KDELR1and KDELR2 proteins. Two transcript variants of KDELR3 that ariseby alternative splicing, and encode different isoforms of KDELR3receptor, have been described.
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :Collins, J.E., et al. Genome Res. 13(1):27-36(2003)Dunham, I., et al. Nature 402(6761):489-495(1999)Pelham, H.R. Cell Struct. Funct. 21(5):413-419(1996)
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