|Other Names||Protein Hook homolog 3, h-hook3, hHK3, HOOK3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13225c was selected from the Center region of HOOK3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non- processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).|
|Cellular Location||Cytoplasm, cytoskeleton. Golgi apparatus. Note=Enriched at the cis-face of the Golgi complex. Localizes to microtubule asters in prophase|
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Provided below are standard protocols that you may find useful for product applications.
Hook proteins are cytosolic coiled-coil proteins thatcontain conserved N-terminal domains, which attach to microtubules,and more divergent C-terminal domains, which mediate binding toorganelles. The Drosophila Hook protein is a component of theendocytic compartment.
Ge, X., et al. Neuron 65(2):191-203(2010)Xu, L., et al. Mol. Biol. Cell 19(12):5059-5071(2008)Ciampi, R., et al. Endocr. Relat. Cancer 14(2):445-452(2007)Sano, H., et al. J. Biol. Chem. 282(11):7973-7981(2007)Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
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