|Other Names||Histone deacetylase 1, HD1, HDAC1, RPD3L1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13296a was selected from the N-term region of HDAC1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.|
|Tissue Location||Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain|
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Provided below are standard protocols that you may find useful for product applications.
Histone acetylation and deacetylation, catalyzed bymultisubunit complexes, play a key role in the regulation ofeukaryotic gene expression. The protein encoded by this genebelongs to the histone deacetylase/acuc/apha family and is acomponent of the histone deacetylase complex. It also interactswith retinoblastoma tumor-suppressor protein and this complex is akey element in the control of cell proliferation anddifferentiation. Together with metastasis-associated protein-2, itdeacetylates p53 and modulates its effect on cell growth andapoptosis.
Yang, Z., et al. Clin. Chem. Lab. Med. 48(12):1785-1791(2010)Grausenburger, R., et al. J. Immunol. 185(6):3489-3497(2010)Miller, K.M., et al. Nat. Struct. Mol. Biol. 17(9):1144-1151(2010)Brandt, S., et al. Int. J. Biochem. Cell Biol. 42(9):1472-1481(2010)Leone, V., et al. Oncogene 29(30):4341-4351(2010)
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