|Other Names||Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO3, FKHRL1, FOXO3A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13306a was selected from the N-term region of FOXO3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' (PubMed:21329882). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301).|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Mitochondrion matrix Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Retention in the cytoplasm contributes to its inactivation (PubMed:10102273, PubMed:15084260, PubMed:16751106). Translocates to the nucleus upon oxidative stress and in the absence of survival factors (PubMed:10102273, PubMed:16751106). Translocates in a AMPK- dependent manner into the mitochondrion in response to metabolic stress (PubMed:23283301, PubMed:29445193)|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the forkhead family of transcriptionfactors which are characterized by a distinct forkhead domain. Thisgene likely functions as a trigger for apoptosis through expressionof genes necessary for cell death. Translocation of this gene withthe MLL gene is associated with secondary acute leukemia.Alternatively spliced transcript variants encoding the same proteinhave been observed.
Zhuo de, X., et al. J. Biol. Chem. 285(41):31491-31501(2010)Tudzarova, S., et al. EMBO J. 29(19):3381-3394(2010)Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Mikse, O.R., et al. Cancer Res. 70(15):6205-6215(2010)Chen, J., et al. PLoS ONE 5 (8), E12293 (2010) :
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