|Other Names||E3 ubiquitin-protein ligase MARCH4, 632-, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, MARCH-IV, RING finger protein 174, MARCH4, KIAA1399, RNF174|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13332b was selected from the C-term region of 40606. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.|
|Cellular Location||Golgi apparatus membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in brain and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
MARCH4 is a member of the MARCH family of membrane-boundE3 ubiquitin ligases (EC 188.8.131.52). MARCH enzymes add ubiquitin(see MIM 191339) to target lysines in substrate proteins, therebysignaling their vesicular transport between membrane compartments.MARCH4 reduces surface accumulation of several membraneglycoproteins by directing them to the endosomal compartment(Bartee et al., 2004 [PubMed 14722266]).
Bartee, E., et al. J. Virol. 78(3):1109-1120(2004)
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