CHST15 Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q7LFX5 |
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Clone Names | 100324264 |
Gene ID | 51363 |
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Other Names | Carbohydrate sulfotransferase 15, B-cell RAG-associated gene protein, hBRAG, N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, CHST15, BRAG, GALNAC4S6ST, KIAA0598 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13376b was selected from the C-term region of CHST15. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | CHST15 |
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Synonyms | BRAG, GALNAC4S6ST, KIAA0598 |
Function | Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. |
Cellular Location | Golgi apparatus membrane; Single-pass type II membrane protein. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor |
Tissue Location | Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T- cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. |
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Background
Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
References
Olsen, J.V., et al. Cell 127(3):635-648(2006)Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)Sawada, T., et al. Carbohydr. Res. 340(12):1983-1996(2005)Deloukas, P., et al. Nature 429(6990):375-381(2004)Ohtake, S., et al. J. Biol. Chem. 278(40):38443-38452(2003)
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