|Other Names||Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1, 3-OST-1, Heparan sulfate 3-O-sulfotransferase 1, h3-OST-1, HS3ST1, 3OST, 3OST1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13378b was selected from the C-term region of HS3ST1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.|
|Cellular Location||Golgi apparatus lumen.|
|Tissue Location||Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta|
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Provided below are standard protocols that you may find useful for product applications.
Heparan sulfate biosynthetic enzymes are key components ingenerating a myriad of distinct heparan sulfate fine structuresthat carry out multiple biologic activities. The enzyme encoded bythis gene is a member of the heparan sulfate biosynthetic enzymefamily. It possesses both heparan sulfate glucosaminyl3-O-sulfotransferase activity, anticoagulant heparan sulfateconversion activity, and is a rate limiting enzyme for synthesis ofanticoagulant heparan. This enzyme is an intraluminal Golgiresident protein.
Lamesch, P., et al. Genomics 89(3):307-315(2007)Edavettal, S.C., et al. Biochemistry 43(16):4680-4688(2004)Hernaiz, M., et al. Biochem. Biophys. Res. Commun. 276(1):292-297(2000)Liu, J., et al. J. Biol. Chem. 274(8):5185-5192(1999)Shworak, N.W., et al. J. Biol. Chem. 274(8):5170-5184(1999)
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