|Other Names||NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M, NEDD4 WW domain-binding protein 5, Putative MAPK-activating protein PM13, Putative NF-kappa-B-activating protein 164, Putative NFKB and MAPK-activating protein, NDFIP1, N4WBP5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13449c was selected from the Center region of NDFIP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.|
|Cellular Location||Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Secreted Note=Detected in exosomes and secreted via the exosomal pathway|
|Tissue Location||Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene belongs to a small groupof evolutionarily conserved proteins with three transmembranedomains. It is a potential target for ubiquitination by the Nedd4family of proteins. This protein is thought to be part of a familyof integral Golgi membrane proteins.
Mund, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11429-11434(2010)Howitt, J., et al. Proc. Natl. Acad. Sci. U.S.A. 106(36):15489-15494(2009)Rapley, E.A., et al. Nat. Genet. 41(7):807-810(2009)Mund, T., et al. EMBO Rep. 10(5):501-507(2009)Putz, U., et al. J. Biol. Chem. 283(47):32621-32627(2008)
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