|Other Names||E3 ubiquitin-protein ligase pellino homolog 3, Pellino-3, 632-, PELI3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13453a was selected from the N-term region of PELI3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation.|
|Tissue Location||Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung|
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Provided below are standard protocols that you may find useful for product applications.
Toll-like receptors (TLRs; see MIM 603030) and IL1R(IL1R1; MIM 147810) are part of the innate immune response aimed atmobilizing defense mechanisms in response in infection or injury.Pellino proteins, such as PELI3, are intermediate components in thesignaling cascades initiated by TLRs and IL1R (Jensen andWhitehead, 2003 [PubMed 12874243]).
Xiao, H., et al. J. Biol. Chem. 283(21):14654-14664(2008)Butler, M.P., et al. J. Biol. Chem. 280(30):27759-27768(2005)Jensen, L.E., et al. J. Immunol. 171(3):1500-1506(2003)
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