|Other Names||Ragulator complex protein LAMTOR1, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1, Lipid raft adaptor protein p18, Protein associated with DRMs and endosomes, p27Kip1-releasing factor from RhoA, p27RF-Rho, LAMTOR1, C11orf59, PDRO|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13455a was selected from the N-term region of C11orf59. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.|
|Cellular Location||Late endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Cell membrane|
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Provided below are standard protocols that you may find useful for product applications.
C11orf59 activates RHOA. Binds to CDKN1B and prevents interaction of CDKN1B and RHOA. This leaves RHOA in a form accessible to ARHGEF2, thereby promoting ARHGEF2-mediated exchange of GDP for GTP.
Sancak, Y., et al. Cell 141(2):290-303(2010)Guillaumot, P., et al. PLoS ONE 5 (6), E10977 (2010) :Hoshino, D., et al. J. Biol. Chem. 284(40):27315-27326(2009)Nada, S., et al. EMBO J. 28(5):477-489(2009)Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
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