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EIF2S1 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P05198 |
|---|---|
| Clone Names | 100409016 |
| Gene ID | 1965 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13469a was selected from the N-term region of EIF2S1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | EIF2S1 (HGNC:3265) |
|---|---|
| Synonyms | EIF2A |
| Function | Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed:16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed:16289705). EIF2S1/ component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap- dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1- mediated reprogramming (PubMed:19131336, PubMed:33384352). |
| Cellular Location | Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q6ZWX6}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P56286}. Note=Colocalizes with NANOS3 in the stress granules. {ECO:0000250|UniProtKB:Q6ZWX6} |
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Provided below are standard protocols that you may find useful for product applications.
Background
The translation initiation factor EIF2 catalyzes the firstregulated step of protein synthesis initiation, promoting thebinding of the initiator tRNA to 40S ribosomal subunits. Bindingoccurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2is composed of 3 nonidentical subunits, the 36-kD EIF2-alphasubunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908),and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate offormation of the ternary complex is modulated by thephosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed2948954]).
References
Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :Sreejith, R.K., et al. Biochem. Biophys. Res. Commun. 390(2):273-279(2009)
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