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NLRP6 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
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- BACKGROUND
| Primary Accession | P59044 |
|---|---|
| Clone Names | 100422080 |
| Gene ID | 171389 |
|---|---|
| Other Names | NACHT, LRR and PYD domains-containing protein 6, Angiotensin II/vasopressin receptor, PYRIN-containing APAF1-like protein 5, NLRP6, NALP6, PYPAF5 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13529a was selected from the N-term region of NLRP6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | NLRP6 {ECO:0000303|PubMed:21088234, ECO:0000312|HGNC:HGNC:22944} |
|---|---|
| Function | Acts as the sensor component of the NLRP6 inflammasome, which mediates inflammasome activation in response to various pathogen- associated signals, leading to maturation and secretion of IL1B and IL18 (PubMed:30392956, PubMed:34678144). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (PubMed:30674671). Acts as a recognition receptor (PRR): recognizes and binds specific pathogens and other damage-associated signals, such as lipoteichoic acid (LTA), a cell-wall component of Gram-positive bacteria, or double stranded RNA (dsRNA) (PubMed:30392956, PubMed:34678144, PubMed:33377178). May also recognize and bind lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria; however, LPS is probably not a major activator of the NLRP6 inflammasome (PubMed:31932628, PubMed:34678144). Following LTA- or dsRNA-binding, NLRP6 undergoes liquid-liquid phase separation (LLPS), enhancing multivalent interactions, an essential step for the formation of the NLRP6 inflammasome polymeric complex (PubMed:34678144). The NLRP6 inflammasome acts by promoting recruitment of effector pro-inflammatory caspases (CASP1 and/or CASP4) that catalyze maturation and secretion of IL1B and IL18 in the extracellular milieu (PubMed:30674671, PubMed:12387869, PubMed:30392956, PubMed:34678144). The NLRP6 inflammasome plays a central role in the maintenance of epithelial integrity and host defense against microbial infections in the intestine (PubMed:30392956). Required to restrict infection against Gram-positive bacteria by recognizing lipoteichoic acid (LTA), leading to recruitment of CASP4 and CASP1, and subsequent maturation and secretion of IL1B and IL18 (PubMed:30392956, PubMed:33377178). Involved in intestinal antiviral innate immunity together with DHX15: recognizes and binds viral dsRNA to restrict infection by enteric viruses through the interferon pathway and GSDMD-dependent release of IL18 (PubMed:34678144, PubMed:34161762). Required to prevent infection by the apicomplexan parasite Cryptosporidium in enterocytes by promoting GSDMD-dependent release of IL18 (By similarity). The NLRP6 inflammasome may also regulate the gut microbiota composition by acting as a sensor of microbiota-associated metabolites to form a PYCARD/ASC-dependent inflammasome for downstream IL18 release and secretion of antimicrobial peptides (By similarity). Essential for gut mucosal self-renewal and proliferation (By similarity). Regulate mucus secretion in an inflammasome- and autophagy-dependent manner to prevent invasion by enteric bacteria, (By similarity). During systemic bacterial infections, the NLRP6 inflammasome negatively regulates neutrophil recruitment and neutrophil extracellular traps (NETs) formation (By similarity). May promote peripheral nerve recovery following injury via an inflammasome-independent mechanism (By similarity). |
| Cellular Location | Cytoplasm, cytosol. Inflammasome. Cell membrane {ECO:0000250|UniProtKB:Q63035}. Nucleus membrane {ECO:0000250|UniProtKB:Q63035} |
| Tissue Location | Expressed in peripheral blood leukocytes, predominantly in granulocytes and, at lower levels, in CD4(+) and CD8(+) T-cells (PubMed:12387869). Expressed in colonic myofibroblasts (at protein level) (PubMed:21593405). |
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Provided below are standard protocols that you may find useful for product applications.
Background
NALPs are cytoplasmic proteins that form a subfamilywithin the larger CATERPILLER protein family. Most short NALPs,such as NALP6, have an N-terminal pyrin (MEFV; MIM 608107) domain(PYD), followed by a NACHT domain, a NACHT-associated domain (NAD),and a C-terminal leucine-rich repeat (LRR) region. The long NALP,NALP1 (MIM 606636), also has a C-terminal extension containing afunction to find domain (FIIND) and a caspase recruitment domain(CARD). NALPs are implicated in the activation of proinflammatorycaspases (e.g., CASP1; MIM 147678) via their involvement inmultiprotein complexes called inflammasomes (Tschopp et al., 2003[PubMed 12563287]).
References
Cummings, J.R., et al. Tissue Antigens 76(1):48-56(2010)Davila, S., et al. Genes Immun. 11(3):232-238(2010)Albrecht, M., et al. FEBS Lett. 538 (1-3), 173-177 (2003) :Tschopp, J., et al. Nat. Rev. Mol. Cell Biol. 4(2):95-104(2003)Grenier, J.M., et al. FEBS Lett. 530 (1-3), 73-78 (2002) :
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