|Other Names||C->U-editing enzyme APOBEC-1, 354-, Apolipoprotein B mRNA-editing enzyme 1, HEPR, APOBEC1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1352a was selected from the N-term region of human Apobec1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.|
|Tissue Location||Expressed exclusively in the small intestine.|
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Provided below are standard protocols that you may find useful for product applications.
APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36-amino acid peptide. The exon 2-skipped transcript accounts for approximately 50% of APOBEC1 mRNA in the adult small intestine and up to 90% of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.
Blanc, V., et al., J. Biol. Chem. 278(42):41198-41204 (2003).Chester, A., et al., EMBO J. 22(15):3971-3982 (2003).Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003).Mukhopadhyay, D., et al., Am. J. Hum. Genet. 70(1):38-50 (2002).Dance, G.S., et al., J. Biol. Chem. 277(15):12703-12709 (2002).
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