|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13532b was selected from the C-term region of TNF. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Tumor necrosis factor, soluble form: Secreted C-domain 2: Secreted.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a multifunctional proinflammatorycytokine that belongs to the tumor necrosis factor (TNF)superfamily. This cytokine is mainly secreted by macrophages. Itcan bind to, and thus functions through its receptorsTNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in theregulation of a wide spectrum of biological processes includingcell proliferation, differentiation, apoptosis, lipid metabolism,and coagulation. This cytokine has been implicated in a variety ofdiseases, including autoimmune diseases, insulin resistance, andcancer. Knockout studies in mice also suggested the neuroprotectivefunction of this cytokine.
Majewski, P.M., et al. J. Biol. Chem. 285(45):34828-34838(2010)Kwon, H.J., et al. J. Immunol. 185(7):3980-3989(2010)Muller, T., et al. Scand. J. Immunol. 72(4):365-371(2010)Qin, H., et al. J. Int. Med. Res. 38(3):760-768(2010)Acharyya, S., et al. PLoS ONE 5 (8), E12479 (2010) :
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