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NFE2 Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q16621 |
|---|---|
| Clone Names | 100318135 |
| Gene ID | 4778 |
|---|---|
| Other Names | Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45 NF-E2, NFE2 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13561b was selected from the C-term region of NFE2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | NFE2 |
|---|---|
| Function | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1- like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. |
| Cellular Location | Nucleus, PML body. Cytoplasm. Note=The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs) Translocated to the cytoplasm through interaction with ITCH |
| Tissue Location | Expressed in hematopoietic cells and also in colon and testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
References
Wang, W., et al. Blood 116(2):254-266(2010)Zhou, Z., et al. J. Biol. Chem. 285(21):15894-15905(2010)Motohashi, H., et al. Blood 115(3):677-686(2010)Lee, T.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(1):52-57(2010)Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
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