|Other Names||U11/U12 small nuclear ribonucleoprotein 25 kDa protein, U11/U12 snRNP 25 kDa protein, U11/U12-25K, Minus-99 protein, SNRNP25, C16orf33|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13582b was selected from the C-term region of SNR25. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Two types of spliceosomes catalyze splicing of pre-mRNAs.The major U2-type spliceosome is found in all eukaryotes andremoves U2-type introns, which represent more than 99% of pre-mRNAintrons. The minor U12-type spliceosome is found in some eukaryotesand removes U12-type introns, which are rare and have distinctsplice consensus signals. The U12-type spliceosome consists ofseveral small nuclear RNAs and associated proteins. This geneencodes a 25K protein that is a component of the U12-typespliceosome.
Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008)De Gobbi, M., et al. Science 312(5777):1215-1217(2006)Martin, J., et al. Nature 432(7020):988-994(2004)Will, C.L., et al. RNA 10(6):929-941(2004)
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