|Other Names||Prolyl 4-hydroxylase subunit alpha-3, 4-PH alpha-3, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-3, P4HA3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13685c was selected from the Center region of P4HA3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.|
|Cellular Location||Endoplasmic reticulum lumen.|
|Tissue Location||Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions|
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This gene encodes a component of prolyl 4-hydroxylase, akey enzyme in collagen synthesis composed of two identical alphasubunits and two beta subunits. The encoded protein is one ofseveral different types of alpha subunits and provides the majorpart of the catalytic site of the active enzyme. In collagen andrelated proteins, prolyl 4-hydroxylase catalyzes the formation of4-hydroxyproline that is essential to the proper three-dimensionalfolding of newly synthesized procollagen chains. Alternativelyspliced transcript variants have been observed, but theirfull-length nature has not been determined.
Rose, J. Phd, et al. Mol. Med. (2010) In press :Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)Kukkola, L., et al. J. Biol. Chem. 278(48):47685-47693(2003)Van Den Diepstraten, C., et al. Circulation 108(5):508-511(2003)
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