|Other Names||72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, PEX, MMP2, CLG4A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13693c was selected from the Center region of MMP2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.|
|Cellular Location||Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes|
|Tissue Location||Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.|
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Proteins of the matrix metalloproteinase (MMP) family areinvolved in the breakdown of extracellular matrix in normalphysiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in diseaseprocesses, such as arthritis and metastasis. Most MMP's aresecreted as inactive proproteins which are activated when cleavedby extracellular proteinases. This gene encodes an enzyme whichdegrades type IV collagen, the major structural component ofbasement membranes. The enzyme plays a role in endometrialmenstrual breakdown, regulation of vascularization and theinflammatory response. Mutations in this gene have been associatedwith Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO)syndrome. Two transcript variants encoding different isoforms havebeen found for this gene.
Beshir, A.B., et al. Cancer Lett. 299(2):137-149(2010)Alakus, H., et al. World J Surg 34(12):2853-2859(2010)Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)Mossbock, G., et al. Mol. Vis. 16, 1764-1770 (2010) :
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