|Other Names||Collagenase 3, 3424-, Matrix metalloproteinase-13, MMP-13, MMP13|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13706c was selected from the Center region of MMP13. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.|
|Cellular Location||Secreted, extracellular space, extracellular matrix. Secreted|
|Tissue Location||Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.|
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Provided below are standard protocols that you may find useful for product applications.
Proteins of the matrix metalloproteinase (MMP) family areinvolved in the breakdown of extracellular matrix in normalphysiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in diseaseprocesses, such as arthritis and metastasis. Most MMP's aresecreted as inactive proproteins which are activated when cleavedby extracellular proteinases. The protein encoded by this genecleaves type II collagen more efficiently than types I and III. Itmay be involved in articular cartilage turnover and cartilagepathophysiology associated with osteoarthritis. The gene is part ofa cluster of MMP genes which localize to chromosome 11q22.3.
Miranda, K.J., et al. J. Biol. Chem. 285(41):31517-31524(2010)Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010)Yamada, T., et al. Anticancer Res. 30(7):2693-2699(2010)Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
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