|Other Names||Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13754a was selected from the N-term region of SF3B1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.|
|Cellular Location||Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes subunit 1 of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence independent manner and may anchor the U2 snRNP to thepre-mRNA. Splicing factor 3b is also a component of the minorU12-type spliceosome. The carboxy-terminal two-thirds of subunit 1have 22 non-identical, tandem HEAT repeats that form rod-like,helical structures. Alternative splicing results in multipletranscript variants encoding different isoforms. [provided byRefSeq].
Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)Tanuma, N., et al. J. Biol. Chem. 283(51):35805-35814(2008)Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008)Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)Avila, M.L., et al. Biochem. Biophys. Res. Commun. 364(1):26-32(2007)
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