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SF3B1 Antibody (N-term) Blocking peptide

Synthetic peptide

Product Information
Primary Accession O75533
Clone Names 100427219
Peptide ID 100427219
Additional Information
Gene ID 23451
Other Names Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155, SF3B1, SAP155
Target/Specificity The synthetic peptide sequence used to generate the antibody AP13754a was selected from the N-term region of SF3B1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name SF3B1
Synonyms SAP155
Function Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Cellular Location Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm
Research Areas
Citations (0)

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This gene encodes subunit 1 of the splicing factor 3bprotein complex. Splicing factor 3b, together with splicing factor3a and a 12S RNA unit, forms the U2 small nuclearribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3acomplex binds pre-mRNA upstream of the intron's branch site in asequence independent manner and may anchor the U2 snRNP to thepre-mRNA. Splicing factor 3b is also a component of the minorU12-type spliceosome. The carboxy-terminal two-thirds of subunit 1have 22 non-identical, tandem HEAT repeats that form rod-like,helical structures. Alternative splicing results in multipletranscript variants encoding different isoforms. [provided byRefSeq].


Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)Tanuma, N., et al. J. Biol. Chem. 283(51):35805-35814(2008)Pessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008)Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)Avila, M.L., et al. Biochem. Biophys. Res. Commun. 364(1):26-32(2007)

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$ 80.00
Cat# BP13754a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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