|Other Names||Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13755a was selected from the N-term region of MBD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.|
|Cellular Location||Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes|
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Provided below are standard protocols that you may find useful for product applications.
DNA methylation is the major modification of eukaryoticgenomes and plays an essential role in mammalian development. Humanproteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family ofnuclear proteins related by the presence in each of a methyl-CpGbinding domain (MBD). However, unlike the other family members,MBD3 is not capable of binding to methylated DNA. The predictedMBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) andmouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complexcontaining nucleosome remodeling and histone deacetylaseactivities. MBD3 mediates the association of metastasis-associatedprotein 2 (MTA2) with the core histone deacetylase complex.
Bachmann, N., et al. Eur J Med Genet 53(1):23-24(2010)Noh, E.J., et al. Biochem. Biophys. Res. Commun. 378(3):332-337(2009)Morey, L., et al. Mol. Cell. Biol. 28(19):5912-5923(2008)Brown, S.E., et al. Gene 420(2):99-106(2008)Spensberger, D., et al. Biochemistry 47(24):6418-6426(2008)
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