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AMBP Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P02760 |
|---|---|
| Clone Names | 100427278 |
| Gene ID | 259 |
|---|---|
| Other Names | Protein AMBP, Alpha-1-microglobulin, Protein HC, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin, AMBP, HCP, ITIL |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13764a was selected from the N-term region of AMBP. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | AMBP |
|---|---|
| Synonyms | HCP, ITIL |
| Function | [Alpha-1-microglobulin]: Antioxidant and tissue repair protein with reductase, heme-binding and radical-scavenging activities. Removes and protects against harmful oxidants and repairs macromolecules in intravascular and extravascular spaces and in intracellular compartments (PubMed:11877257, PubMed:15683711, PubMed:22096585, PubMed:23157686, PubMed:23642167, PubMed:25698971, PubMed:32823731, PubMed:32092412). Intravascularly, plays a regulatory role in red cell homeostasis by preventing heme- and reactive oxygen species-induced cell damage. Binds and degrades free heme to protect fetal and adult red blood cells from hemolysis (PubMed:11877257, PubMed:32092412). Reduces extracellular methemoglobin, a Fe3+ (ferric) form of hemoglobin that cannot bind oxygen, back to the Fe2+ (ferrous) form deoxyhemoglobin, which has oxygen-carrying potential (PubMed:15683711). Upon acute inflammation, inhibits oxidation of low- density lipoprotein particles by MPO and limits vascular damage (PubMed:25698971). Extravascularly, protects from oxidation products formed on extracellular matrix structures and cell membranes. Catalyzes the reduction of carbonyl groups on oxidized collagen fibers and preserves cellular and extracellular matrix ultrastructures (PubMed:23642167, PubMed:22096585). Importantly, counteracts the oxidative damage at blood-placenta interface, preventing leakage of free fetal hemoglobin into the maternal circulation (PubMed:21356557). Intracellularly, has a role in maintaining mitochondrial redox homeostasis. Bound to complex I of the respiratory chain of mitochondria, may scavenge free radicals and preserve mitochondrial ATP synthesis. Protects renal tubule epithelial cells from heme-induced oxidative damage to mitochondria (PubMed:23157686, PubMed:32823731). Reduces cytochrome c from Fe3+ (ferric) to the Fe2+ (ferrous) state through formation of superoxide anion radicals in the presence of ascorbate or NADH/NADPH electron donor cofactors, ascorbate being the preferred cofactor (PubMed:15683711). Has a chaperone role in facilitating the correct folding of bikunin in the endoplasmic reticulum compartment (By similarity). |
| Cellular Location | [Alpha-1-microglobulin]: Secreted. Endoplasmic reticulum. Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Nucleus membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Secreted, extracellular space, extracellular matrix. Note=The cellular uptake occurs via a non-endocytotic pathway and allows for localization to various membrane structures. A specific binding to plasma membrane suggests the presence of a cell receptor, yet to be identified Directly binds collagen fibers type I. |
| Tissue Location | [Alpha-1-microglobulin]: Expressed by the liver and secreted in plasma. Occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid (PubMed:11877257). Expressed in epidermal keratinocytes, in dermis and epidermal-dermal junction (at protein level) (PubMed:22096585). Expressed in red blood cells (at protein level) (PubMed:32092412). Expressed in placenta (PubMed:21356557). |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a complex glycoprotein secreted inplasma. The precursor is proteolytically processed into distinctfunctioning proteins: alpha-1-microglobulin, which belongs to thesuperfamily of lipocalin transport proteins and may play a role inthe regulation of inflammatory processes, and bikunin, which is aurinary trypsin inhibitor belonging to the superfamily ofKunitz-type protease inhibitors and plays an important role in manyphysiological and pathological processes. This gene is located onchromosome 9 in a cluster of lipocalin genes.
References
Olsson, M.G., et al. Radiat. Res. 174(5):590-600(2010)Allhorn, M., et al. Blood 99(6):1894-1901(2002)Amoresano, A., et al. Eur. J. Biochem. 267(7):2105-2112(2000)Xu, Y., et al. J. Mol. Biol. 276(5):955-966(1998)Vetr, H., et al. Biol. Chem. Hoppe-Seyler 371(12):1185-1196(1990)
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