|Other Names||Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13776a was selected from the N-term region of RANGAP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.|
|Cellular Location||Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation|
|Tissue Location||Highly expressed in brain, thymus and testis.|
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Provided below are standard protocols that you may find useful for product applications.
RanGAP1, is a homodimeric 65-kD polypeptide thatspecifically induces the GTPase activity of RAN, but not of RAS byover 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, aregulator molecule that keeps RAN in the active, GTP-bound state.The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequenceis unrelated to that of GTPase activators for other RAS-relatedproteins, but is 88% identical to Fug1, the murine homolog of yeastRna1p. RanGAP1 and RCC1 control RAN-dependent transport between thenucleus and cytoplasm. RanGAP1 is a key regulator of the RANGTP/GDP cycle.
Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)Zuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
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