|Other Names||Ras GTPase-activating protein-binding protein 1, G3BP-1, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13785b was selected from the C-term region of G3BP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes one of the DNA-unwinding enzymes whichprefers partially unwound 3'-tailed substrates and can also unwindpartial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.This enzyme is a member of the heterogeneous nuclear RNA-bindingproteins and is also an element of the Ras signal transductionpathway. It binds specifically to the Ras-GTPase-activating proteinby associating with its SH3 domain. Several alternatively splicedtranscript variants of this gene have been described, but thefull-length nature of some of these variants has not beendetermined.
Gao, X., et al. FEBS Lett. 584(16):3525-3532(2010)Ortega, A.D., et al. J. Cell. Sci. 123 (PT 16), 2685-2696 (2010) :Hinton, S.D., et al. Biochem. J. 427(3):349-357(2010)Shim, J.H., et al. Cancer Prev Res (Phila) 3(5):670-679(2010)Zhang, H.Z., et al. World J. Gastroenterol. 13(30):4126-4130(2007)
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