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MGAT5 Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q09328 |
|---|---|
| Clone Names | 100318153 |
| Gene ID | 4249 |
|---|---|
| Other Names | Alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, Alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase, GlcNAc-T V, GNT-V, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5, GGNT5 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13815b was selected from the C-term region of MGAT5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | MGAT5 |
|---|---|
| Synonyms | GGNT5 |
| Function | Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed:10395745, PubMed:30140003). Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (PubMed:10395745, PubMed:22614033, PubMed:30140003). Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N- glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (PubMed:22614033). |
| Cellular Location | Golgi apparatus membrane {ECO:0000250|UniProtKB:P97259}; Single-pass type II membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes mannosyl (alpha-1,6-)-glycoproteinbeta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferaseinvolved in the synthesis of protein-bound and lipid-boundoligosaccharides. Alterations of the oligosaccharides on cellsurface glycoproteins cause significant changes in the adhesive ormigratory behavior of a cell. Increase in the encoded protein'sactivity may correlate with the progression of invasivemalignancies.
References
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :Brynedal, B., et al. J. Neuroimmunol. 220 (1-2), 120-124 (2010) :Benson, V., et al. Int. Immunol. 22(3):167-177(2010)Wang, C., et al. J. Cell. Biochem. 109(1):113-123(2010)Ding, H., et al. Stroke 41(1):177-180(2010)
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