|Other Names||Ubiquitin-conjugating enzyme E2 E2, UbcH8, Ubiquitin carrier protein E2, Ubiquitin-protein ligase E2, UBE2E2, UBCH8|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13824a was selected from the N-term region of UBE2E2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.|
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Provided below are standard protocols that you may find useful for product applications.
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
Yamauchi, T., et al. Nat. Genet. 42(10):864-868(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)Ng, D., et al. Int. J. Cancer 123(7):1610-1615(2008)Takeuchi, T., et al. J. Biochem. 138(6):711-719(2005)
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