|Other Names||Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13843c was selected from the Center region of CENPA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).|
|Cellular Location||Nucleus Chromosome, centromere, kinetochore Chromosome, centromere. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658).|
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Provided below are standard protocols that you may find useful for product applications.
Centromeres are the differentiated chromosomal domainsthat specify the mitotic behavior of chromosomes. CENPA encodes acentromere protein which contains a histone H3 related histone folddomain that is required for targeting to the centromere. CENPA isproposed to be a component of a modified nucleosome ornucleosome-like structure in which it replaces 1 or both copies ofconventional histone H3 in the (H3-H4)2 tetrameric core of thenucleosome particle. Alternative splicing results in multipletranscript variants encoding distinct isoforms. [provided byRefSeq].
Sekulic, N., et al. Nature 467(7313):347-351(2010)Carroll, C.W., et al. J. Cell Biol. 189(7):1143-1155(2010)Maehara, K., et al. Mol. Cell. Biol. 30(9):2090-2104(2010)Buscaino, A., et al. Curr. Opin. Genet. Dev. 20(2):118-126(2010)Pironon, N., et al. BMC Genomics 11, 195 (2010) :
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