|Other Names||Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13843c was selected from the Center region of CENPA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292).|
|Cellular Location||Nucleus Chromosome, centromere, kinetochore Chromosome, centromere. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658).|
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Centromeres are the differentiated chromosomal domainsthat specify the mitotic behavior of chromosomes. CENPA encodes acentromere protein which contains a histone H3 related histone folddomain that is required for targeting to the centromere. CENPA isproposed to be a component of a modified nucleosome ornucleosome-like structure in which it replaces 1 or both copies ofconventional histone H3 in the (H3-H4)2 tetrameric core of thenucleosome particle. Alternative splicing results in multipletranscript variants encoding distinct isoforms. [provided byRefSeq].
Sekulic, N., et al. Nature 467(7313):347-351(2010)Carroll, C.W., et al. J. Cell Biol. 189(7):1143-1155(2010)Maehara, K., et al. Mol. Cell. Biol. 30(9):2090-2104(2010)Buscaino, A., et al. Curr. Opin. Genet. Dev. 20(2):118-126(2010)Pironon, N., et al. BMC Genomics 11, 195 (2010) :
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