|Other Names||Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13849b was selected from the C-term region of RARA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation|
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Provided below are standard protocols that you may find useful for product applications.
This gene represents a nuclear retinoic acid receptor. Theencoded protein, retinoic acid receptor alpha, regulatestranscription in a ligand-dependent manner. This gene has beenimplicated in regulation of development, differentiation,apoptosis, granulopoeisis, and transcription of clock genes.Translocations between this locus and several other loci have beenassociated with acute promyelocytic leukemia. Alternatively splicedtranscript variants have been found for this locus.
Park, U.H., et al. J. Biol. Chem. 285(44):34269-34278(2010)Asleson, A.D., et al. Cancer Genet. Cytogenet. 202(1):33-37(2010)Catalano, A., et al. Blood 110(12):4073-4076(2007)Wells, R.A., et al. Nat. Genet. 17(1):109-113(1997)Chen, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 91(3):1178-1182(1994)
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