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MDM4 Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O15151 |
|---|---|
| Clone Names | 100430093 |
| Gene ID | 4194 |
|---|---|
| Other Names | Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4, MDM4, MDMX |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13861c was selected from the Center region of MDM4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | MDM4 |
|---|---|
| Synonyms | MDMX |
| Function | Along with MDM2, contributes to TP53 regulation (PubMed:32300648). Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in all tissues tested with high levels in thymus |
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Provided below are standard protocols that you may find useful for product applications.
Background
The human MDM4 gene, which plays a role in apoptosis,encodes a 490-amino acid protein containing a RING finger domainand a putative nuclear localization signal. The MDM4 putativenuclear localization signal, which all Mdm proteins contain, islocated in the C-terminal region of the protein. The mRNA isexpressed at a high level in thymus and at lower levels in allother tissues tested. MDM4 protein produced by in vitro translationinteracts with p53 via a binding domain located in the N-terminalregion of the MDM4 protein. MDM4 shows significant structuralsimilarity to p53-binding protein MDM2. Two transcript variants,one protein-coding and the other likely not to be protein-coding,have been found for this gene.
References
Xu, N., et al. Biochem. Biophys. Res. Commun. 401(3):417-421(2010)Sarkari, F., et al. J. Mol. Biol. 402(5):825-837(2010)Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Fang, S., et al. PLoS ONE 5 (5), E10813 (2010) :
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