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PPM1D Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | O15297 |
|---|---|
| Clone Names | 100430203 |
| Gene ID | 8493 |
|---|---|
| Other Names | Protein phosphatase 1D, Protein phosphatase 2C isoform delta, PP2C-delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D, WIP1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13875c was selected from the Center region of PPM1D. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | PPM1D |
|---|---|
| Synonyms | WIP1 |
| Function | Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol |
| Tissue Location | Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the PP2Cfamily of Ser/Thr protein phosphatases. PP2C family members areknown to be negative regulators of cell stress response pathways.The expression of this gene is induced in a p53-dependent manner inresponse to various environmental stresses. While being induced bytumor suppressor protein TP53/p53, this phosphatase negativelyregulates the activity of p38 MAP kinase, MAPK/p38, through whichit reduces the phosphorylation of p53, and in turn suppressesp53-mediated transcription and apoptosis. This phosphatase thusmediates a feedback regulation of p38-p53 signaling thatcontributes to growth inhibition and the suppression of stressinduced apoptosis. This gene is located in a chromosomal regionknown to be amplified in breast cancer. The amplification of thisgene has been detected in both breast cancer cell line and primarybreast tumors, which suggests a role of this gene in cancerdevelopment.
References
Zhang, X., et al. Cancer Res. 70(18):7176-7186(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Moon, S.H., et al. J. Biol. Chem. 285(17):12935-12947(2010)Macurek, L., et al. Oncogene 29(15):2281-2291(2010)Yang, D.H., et al. Zhonghua Yi Xue Za Zhi 90(8):519-522(2010)
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