|Other Names||Neuronal acetylcholine receptor subunit alpha-7, CHRNA7, NACHRA7|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13898a was selected from the N-term region of CHRNA7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.|
|Cellular Location||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
The nicotinic acetylcholine receptors (nAChRs) are membersof a superfamily of ligand-gated ion channels that mediate fastsignal transmission at synapses. The nAChRs are thought to behetero-pentamers composed of homologous subunits. The proposedstructure for each subunit is a conserved N-terminal extracellulardomain followed by three conserved transmembrane domains, avariable cytoplasmic loop, a fourth conserved transmembrane domain,and a short C-terminal extracellular region. The protein encoded bythis gene forms a homo-oligomeric channel, displays markedpermeability to calcium ions and is a major component of brainnicotinic receptors that are blocked by, and highly sensitive to,alpha-bungarotoxin. Once this receptor binds acetylcholine, itundergoes an extensive change in conformation that affects allsubunits and leads to opening of an ion-conducting channel acrossthe plasma membrane. This gene is located in a region identified asa major susceptibility locus for juvenile myoclonic epilepsy and achromosomal location involved in the genetic transmission ofschizophrenia. An evolutionarily recent partial duplication eventin this region results in a hybrid containing sequence from thisgene and a novel FAM7A gene. Alternatively spliced transcriptvariants encoding different isoforms have been found for this gene.
Chernyavsky, A.I., et al. Am. J. Physiol., Cell Physiol. 299 (5), C903-C911 (2010) :Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)Jin, Y., et al. Int. J. Immunogenet. (2010) In press :Schraufstatter, I.U., et al. J Stem Cells 4(4):203-215(2009)
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