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Mouse Nlk Antibody (C-term) Blocking peptide

Synthetic peptide

Product Information
Primary Accession O54949
Clone Names 110316297
Peptide ID 110316297
Additional Information
Gene ID 18099
Other Names Serine/threonine-protein kinase NLK, Nemo-like kinase, Nlk {ECO:0000312|EMBL:AAC244991}
Target/Specificity The synthetic peptide sequence used to generate the antibody AP13929b was selected from the C-term region of Mouse Nlk. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nlk {ECO:0000312|EMBL:AAC24499.1}
Function Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic
Tissue Location Expressed at high levels in the brain, and at lower levels in heart, kidney, lung and liver
Research Areas
Citations (0)

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Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.


Li, M., et al. J. Biol. Chem. 285(18):13397-13404(2010)Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010)Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009)Harwood, B.N., et al. Dev. Dyn. 237(4):1099-1111(2008)

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$ 80.00
Cat# BP13929b
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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