|Other Names||Serine/threonine-protein kinase PLK4, Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak, Plk4, Sak, Stk18|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13934b was selected from the C-term region of Mouse Plk4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus Cleavage furrow. Note=Associates with centrioles throughout the cell cycle. According to PubMed:11301255, it localizes to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis. Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles|
|Tissue Location||expressed in tissues associated with mitotic and meiotic cell division. Highly expressed in testis|
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Provided below are standard protocols that you may find useful for product applications.
Plk4 is a member of the polo family ofserine/threonine protein kinases. The protein localizes to thenucleolus during G2, to centrosomes during G2/M, and to thecleavage furrow during cytokinesis. It is required for progressionthrough mitosis, cell survival, and embryonic development. Themouse genome contains a pseudogene similar to this gene.Alternatively spliced transcript variants have been described, buttheir biological validity has not been determined. [provided byRefSeq].
Rosario, C.O., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6888-6893(2010)Holland, A.J., et al. J. Cell Biol. 188(2):191-198(2010)Morettin, A., et al. BMC Genomics 10, 319 (2009) :Martindill, D.M., et al. Nat. Cell Biol. 9(10):1131-1141(2007)Leung, G.C., et al. FEBS Lett. 581(1):77-83(2007)
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