|Other Names||Vasodilator-stimulated phosphoprotein, VASP, VASP|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13999b was selected from the C-term region of VASP. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)|
|Tissue Location||Highly expressed in platelets.|
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Provided below are standard protocols that you may find useful for product applications.
Vasodilator-stimulated phosphoprotein (VASP) is a memberof the Ena-VASP protein family. Ena-VASP family members contain anEHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/Emotifs and targets Ena-VASP proteins to focal adhesions. In themid-region of the protein, family members have a proline-richdomain that binds SH3 and WW domain-containing proteins. TheirC-terminal EVH2 domain mediates tetramerization and binds both Gand F actin. VASP is associated with filamentous actin formationand likely plays a widespread role in cell adhesion and motility.VASP may also be involved in the intracellular signaling pathwaysthat regulate integrin-extracellular matrix interactions. VASP isregulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Barragan, P., et al. Thromb. Haemost. 104(2):410-411(2010)Dittrich, M., et al. Arterioscler. Thromb. Vasc. Biol. 30(4):843-850(2010)Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010)Osmancik, P., et al. Catheter Cardiovasc Interv 75(2):158-166(2010)Siller-Matula, J.M., et al. J. Thromb. Haemost. 8(2):351-359(2010)
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