|Other Names||NADPH oxidase 4, 163-, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, NOX4, RENOX|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14025a was selected from the N-term region of NOX4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus|
|Tissue Location||Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells|
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This gene encodes a member of the NOX-family of enzymesthat functions as the catalytic subunit the NADPH oxidase complex.The encoded protein is localized to non-phagocytic cells where itacts as an oxygen sensor and catalyzes the reduction of molecularoxygen to various reactive oxygen species (ROS). The ROS generatedby this protein have been implicated in numerous biologicalfunctions including signal transduction, cell differentiation andtumor cell growth. A pseudogene has been identified on the otherarm of chromosome 11. Alternative splicing results in multipletranscript variants.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Salmeen, A., et al. Oncogene 29(31):4473-4484(2010)Wu, R.F., et al. Mol. Cell. Biol. 30(14):3553-3568(2010)Diebold, I., et al. Mol. Biol. Cell 21(12):2087-2096(2010)Manea, A., et al. Biochem. Biophys. Res. Commun. 396(4):901-907(2010)
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