|Other Names||DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14026a was selected from the N-term region of MCM3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.|
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The protein encoded by this gene is one of the highlyconserved mini-chromosome maintenance proteins (MCM) that areinvolved in the initiation of eukaryotic genome replication. Thehexameric protein complex formed by MCM proteins is a key componentof the pre-replication complex (pre_RC) and may be involved in theformation of replication forks and in the recruitment of other DNAreplication related proteins. This protein is a subunit of theprotein complex that consists of MCM2-7. It has been shown tointeract directly with MCM5/CDC46. This protein also interactswith, and thus is acetlyated by MCM3AP, a chromatin-associatedacetyltransferase. The acetylation of this protein inhibits theinitiation of DNA replication and cell cycle progression. [providedby RefSeq].
Lau, K.M., et al. Oncogene 29(40):5475-5489(2010)Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :Lee, Y.S., et al. Exp. Mol. Pathol. 88(1):138-142(2010)Song, Y.J., et al. Acta Virol. 54(2):125-130(2010)Saade, E., et al. Proteomics 9(21):4934-4943(2009)
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