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BANF1 Antibody (Center) Blocking peptide

Synthetic peptide

Product Information
Primary Accession O75531
Clone Names 100507272
Peptide ID 100507272
Additional Information
Gene ID 8815
Other Names Barrier-to-autointegration factor, Breakpoint cluster region protein 1, Barrier-to-autointegration factor, N-terminally processed, BANF1, BAF, BCRG1
Target/Specificity The synthetic peptide sequence used to generate the antibody AP14071c was selected from the Center region of BANF1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name BANF1
Synonyms BAF, BCRG1
Function Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Cellular Location Nucleus. Cytoplasm. Chromosome. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization
Tissue Location Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes
Research Areas
Citations (0)

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The protein encoded by this gene was first identified byits ability to protect retroviruses from intramolecular integrationand therefore promote intermolecular integration into the host cellgenome. The protein forms a homodimer which localizes to both thenucleus and cytoplasm and is specifically associated withchromosomes during mitosis. This protein binds to double strandedDNA in a non-specific manner and also binds to LEM-domaincontaining proteins of the nuclear envelope. This protein isthought to facilitate nuclear reassembly by binding with both DNAand inner nuclear membrane proteins and thereby recruit chromatinto the nuclear periphery. Alternative splicing results in multipletranscript variants encoding the same protein.


Montes de Oca, R., et al. PLoS ONE 4 (9), E7050 (2009) :Haraguchi, T., et al. J. Cell. Sci. 121 (PT 15), 2540-2554 (2008) :Haraguchi, T., et al. J. Cell. Sci. 120 (PT 12), 1967-1977 (2007) :Wiebe, M.S., et al. Cell Host Microbe 1(3):187-197(2007)Bengtsson, L., et al. Mol. Biol. Cell 17(3):1154-1163(2006)

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$ 80.00
Cat# BP14071c
Availability: In Stock
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