|Other Names||Nicotinamide mononucleotide adenylyltransferase 3, NMN adenylyltransferase 3, Nicotinate-nucleotide adenylyltransferase 3, NaMN adenylyltransferase 3, Pyridine nucleotide adenylyltransferase 3, PNAT-3, NMNAT3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14087c was selected from the Center region of NMNAT3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.|
|Tissue Location||Expressed in lung and spleen with lower levels in placenta and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
The coenzyme NAD and its derivatives are involved inhundreds of metabolic redox reactions and are utilized in proteinADP-ribosylation, histone deacetylation, and in some Ca(2+)signaling pathways. NMNAT (EC 184.108.40.206) is a central enzyme in NADbiosynthesis, catalyzing the condensation of nicotinamidemononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) withthe AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003[PubMed 12574164]).
Di Stefano, M., et al. Blood Cells Mol. Dis. 45(1):33-39(2010)Lau, C., et al. J. Biol. Chem. 285(24):18868-18876(2010)Sorci, L., et al. Biochemistry 46(16):4912-4922(2007)Berger, F., et al. J. Biol. Chem. 280(43):36334-36341(2005)Magni, G., et al. Cell. Mol. Life Sci. 61(1):19-34(2004)
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