|Other Names||DNA excision repair protein ERCC-1, ERCC1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14106b was selected from the C-term region of ERCC1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1: Non-catalytic component of a structure- specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.|
|Cellular Location||Isoform 1: Nucleus. Isoform 3: Nucleus.|
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The product of this gene functions in the nucleotideexcision repair pathway, and is required for the repair of DNAlesions such as those induced by UV light or formed byelectrophilic compounds including cisplatin. The encoded proteinforms a heterodimer with the XPF endonuclease (also known asERCC4), and the heterodimeric endonuclease catalyzes the 5'incision in the process of excising the DNA lesion. Theheterodimeric endonuclease is also involved in recombinational DNArepair and in the repair of inter-strand crosslinks. Mutations inthis gene result in cerebrooculofacioskeletal syndrome, andpolymorphisms that alter expression of this gene may play a role incarcinogenesis. Multiple transcript variants encoding differentisoforms have been found for this gene. The last exon of this geneoverlaps with the CD3e molecule, epsilon associated protein gene onthe opposite strand.
Palli, D., et al. Mutagenesis 25(6):569-575(2010)Scheil-Bertram, S., et al. Gynecol. Oncol. 119(2):325-331(2010)Wenchao, L., et al. Diagn. Mol. Pathol. 19(3):164-168(2010)Hoffmann, A.C., et al. Neoplasia 12(8):628-636(2010)van Duin, M., et al. Mol. Cell. Biol. 9(4):1794-1798(1989)
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