|Other Names||Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, PNKP|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14116a was selected from the N-term region of PNKP. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.|
|Tissue Location||Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).|
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Provided below are standard protocols that you may find useful for product applications.
This locus represents a gene involved in DNA repair. Inresponse to ionizing radiation or oxidative damage, the proteinencoded by this locus catalyzes 5' phosphorylation and 3'dephosphorylation of nucleic acids. Mutations at this locus havebeen associated with microcephaly, seizures, and developmentaldelay.
Arora, M., et al. Leukemia 24(8):1470-1475(2010)Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)Shen, J., et al. Nat. Genet. 42(3):245-249(2010)Ali, A.A., et al. Nucleic Acids Res. 37(5):1701-1712(2009)
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