|Other Names||Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.|
|Cellular Location||Isoform 2: Nucleus|
|Tissue Location||Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).|
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This gene encodes an essential enzyme of nucleotidemetabolism. The encoded protein forms a ubiquitous, homotetramericenzyme that hydrolyzes dUTP to dUMP and pyrophosphate. Thisreaction serves two cellular purposes: providing a precursor (dUMP)for the synthesis of thymine nucleotides needed for DNAreplication, and limiting intracellular pools of dUTP. Elevatedlevels of dUTP lead to increased incorporation of uracil into DNA,which induces extensive excision repair mediated by uracilglycosylase. This repair process, resulting in the removal andreincorporation of dUTP, is self-defeating and leads to DNAfragmentation and cell death. Alternative splicing of this geneleads to different isoforms that localize to either themitochondrion or nucleus. A related pseudogene is located onchromosome 19.
Takatori, H., et al. Liver Int. 30(3):438-446(2010)Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010)Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009)Takacs, E., et al. FEBS Lett. 583(5):865-871(2009)Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
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