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AKT1S1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q96B36 |
|---|---|
| Clone Names | 100528104 |
| Gene ID | 84335 |
|---|---|
| Other Names | Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1 {ECO:0000312|EMBL:AAH160431} |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | AKT1S1 {ECO:0000312|EMBL:AAH16043.1} |
|---|---|
| Function | Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:17510057, PubMed:17386266, PubMed:17277771, PubMed:29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate- recruitment site (PubMed:29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed:17386266, PubMed:18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed:16174443, PubMed:18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity). |
| Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9D1F4}. Note=Found in the cytosolic fraction of the brain. {ECO:0000250|UniProtKB:Q9D1F4} |
| Tissue Location | Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
AKT1S1 is a proline-rich substrate of AKT (MIM 164730)that binds 14-3-3 protein (see YWHAH, MIM 113508) whenphosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[suppliedby OMIM].
References
Wang, L., et al. J. Biol. Chem. 283(23):15619-15627(2008)Fonseca, B.D., et al. Biochem. J. 411(1):141-149(2008)Fonseca, B.D., et al. J. Biol. Chem. 282(34):24514-24524(2007)Wang, L., et al. J. Biol. Chem. 282(27):20036-20044(2007)Sancak, Y., et al. Mol. Cell 25(6):903-915(2007)
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