|Other Names||Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4, USP15, KIAA0529|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes (PubMed:21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal (PubMed:22344298). Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B (PubMed:24526689). Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitin (MIM 191339), a highly conserved proteininvolved in the regulation of intracellular protein breakdown, cellcycle regulation, and stress response, is released from degradedproteins by disassembly of the polyubiquitin chains. Thedisassembly process is mediated by ubiquitin-specific proteases(USPs). Also see USP1 (MIM 603478).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Vos, R.M., et al. J. Virol. 83(17):8885-8892(2009)Huang, X., et al. J. Mol. Biol. 391(4):691-702(2009)Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
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