|Other Names||E3 ubiquitin-protein ligase BRE1B, BRE1-B, 632-, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.|
|Tissue Location||Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine|
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The protein encoded by this gene contains a RING finger, amotif known to be involved in protein-protein and protein-DNAinteractions. This protein was reported to interact with the tumorsuppressor protein RB1. Studies of the rat counterpart suggestedthat this protein may function as an E3 ubiquitin-protein ligase,and facilitate the ubiquitination and degradation of syntaxin 1,which is an essential component of the neurotransmitter releasemachinery.
Chernikova, S.B., et al. Radiat. Res. 174(5):558-565(2010)Kim, J., et al. Cell 137(3):459-471(2009)Olsen, J.V., et al. Cell 127(3):635-648(2006)Olsen, J.V., et al. Cell 127(3):635-648(2006)Zhu, B., et al. Mol. Cell 20(4):601-611(2005)
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