|Other Names||Protein mago nashi homolog, MAGOH, MAGOHA|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.|
|Cellular Location||Nucleus. Nucleus speckle. Cytoplasm. Note=Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.|
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Drosophila that have mutations in their mago nashi(grandchildless) gene produce progeny with defects in germplasmassembly and germline development. This gene encodes the mammalianmago nashi homolog. In mammals, mRNA expression is not limited tothe germ plasm, but is expressed ubiquitously in adult tissues andcan be induced by serum stimulation of quiescent fibroblasts.
Gehring, N.H., et al. Cell 137(3):536-548(2009)Muromoto, R., et al. Biochem. Biophys. Res. Commun. 382(1):63-68(2009)Diem, M.D., et al. Nat. Struct. Mol. Biol. 14(12):1173-1179(2007)Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :Andersen, C.B., et al. Science 313(5795):1968-1972(2006)
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