|Other Names||DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.|
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The protein encoded by this gene is one of the highlyconserved mini-chromosome maintenance proteins (MCM) that areessential for the initiation of eukaryotic genome replication. Thehexameric protein complex formed by MCM proteins is a key componentof the pre-replication complex (pre_RC) and may be involved in theformation of replication forks and in the recruitment of other DNAreplication related proteins. The MCM complex consisting of thisprotein and MCM2, 6 and 7 proteins possesses DNA helicase activity,and may act as a DNA unwinding enzyme. The phosphorylation of thisprotein by CDC2 kinase reduces the DNA helicase activity andchromatin binding of the MCM complex. This gene is mapped to aregion on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, aDNA-activated protein kinase involved in the repair of DNAdouble-strand breaks. Alternatively spliced transcript variantsencoding the same protein have been reported.
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :Qian, Z., et al. PLoS Pathog. 6 (3), E1000814 (2010) :Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)Enjuanes, A., et al. Cancer Res. 68(24):10178-10186(2008)
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