|Other Names||Laforin, 313-, Glucan phosphatase, Lafora PTPase, LAFPTPase, EPM2A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1453a was selected from the N-term region of human EPM2A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Has both dual-specificity protein phosphatase and glucan phosphatase activities. Together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1.|
|Cellular Location||Cytoplasm Note=Under glycogenolytic conditions localizes to the nucleus Isoform 2: Endoplasmic reticulum. Cell membrane. Nucleus. Note=Also found in the nucleus Isoform 5: Cytoplasm. Nucleus.|
|Tissue Location||Expressed in heart, skeletal muscle, kidney, pancreas and brain. Isoform 4 is also expressed in the placenta|
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Provided below are standard protocols that you may find useful for product applications.
EPM2A is a dual-specificity phosphatase that associates with polyribosomes. This protein may be involved in the regulation of glycogen metabolism. Mutations have been associated with myoclonic epilepsy of Lafora.
Minassian B.A., Nat. Genet. 20:171-174(1998). Ganesh S., Hum. Mol. Genet. 9:2251-2261(2000).
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