|Other Names||Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus Isoform 2: Cytoplasm|
|Tissue Location||Detected in erythrocytes (at protein level). Ubiquitous.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene catalyzes the first stepin ubiquitin conjugation to mark cellular proteins for degradation.This gene complements an X-linked mouse temperature-sensitivedefect in DNA synthesis, and thus may function in DNA repair. It ispart of a gene cluster on chromosome Xp11.23. Alternatively splicedtranscript variants that encode the same protein have beendescribed.
Burkhardt, J., et al. J. Rheumatol. 36(10):2149-2157(2009)Su, Z.L., et al. Leuk. Lymphoma 49(9):1821-1822(2008)Ramser, J., et al. Am. J. Hum. Genet. 82(1):188-193(2008)Carbia-Nagashima, A., et al. Cell 131(2):309-323(2007)Jin, J., et al. Nature 447(7148):1135-1138(2007)
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