|Other Names||Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit, U2AF2, U2AF65|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
U2 auxiliary factor (U2AF), comprised of a large and asmall subunit, is a non-snRNP protein required for the binding ofU2 snRNP to the pre-mRNA branch site. This gene encodes the U2AFlarge subunit which contains a sequence-specific RNA-binding regionwith 3 RNA recognition motifs and an Arg/Ser-rich domain necessaryfor splicing. The large subunit binds to the polypyrimidine tractof introns early during spliceosome assembly. Multiple transcriptvariants have been detected for this gene, but the full-lengthnatures of only two have been determined to date. [provided byRefSeq].
Webby, C.J., et al. Science 325(5936):90-93(2009)Martins de Araujo, M., et al. RNA 15(4):515-523(2009)Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)Jenkins, J.L., et al. J. Biol. Chem. 283(48):33641-33649(2008)Izquierdo, J.M. Mol. Biol. Cell 19(8):3299-3307(2008)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.