|Other Names||Splicing factor U2AF 65 kDa subunit, U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit, U2AF2, U2AF65|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.|
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Provided below are standard protocols that you may find useful for product applications.
U2 auxiliary factor (U2AF), comprised of a large and asmall subunit, is a non-snRNP protein required for the binding ofU2 snRNP to the pre-mRNA branch site. This gene encodes the U2AFlarge subunit which contains a sequence-specific RNA-binding regionwith 3 RNA recognition motifs and an Arg/Ser-rich domain necessaryfor splicing. The large subunit binds to the polypyrimidine tractof introns early during spliceosome assembly. Multiple transcriptvariants have been detected for this gene, but the full-lengthnatures of only two have been determined to date. [provided byRefSeq].
Webby, C.J., et al. Science 325(5936):90-93(2009)Martins de Araujo, M., et al. RNA 15(4):515-523(2009)Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)Jenkins, J.L., et al. J. Biol. Chem. 283(48):33641-33649(2008)Izquierdo, J.M. Mol. Biol. Cell 19(8):3299-3307(2008)
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