MCM6 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q14566 |
---|---|
Clone Names | 100525202 |
Gene ID | 4175 |
---|---|
Other Names | DNA replication licensing factor MCM6, p105MCM, MCM6 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | MCM6 (HGNC:6949) |
---|---|
Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:16899510, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). |
Cellular Location | Nucleus. Chromosome. Note=Binds to chromatin during G1 and detaches from it during S phase. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is one of the highlyconserved mini-chromosome maintenance proteins (MCM) that areessential for the initiation of eukaryotic genome replication. Thehexameric protein complex formed by the MCM proteins is a keycomponent of the pre-replication complex (pre_RC) and may beinvolved in the formation of replication forks and in therecruitment of other DNA replication related proteins. The MCMcomplex consisting of this protein and MCM2, 4 and 7 proteinspossesses DNA helicase activity, and may act as a DNA unwindingenzyme. The phosphorylation of the complex by CDC2 kinase reducesthe helicase activity, suggesting a role in the regulation of DNAreplication.
References
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :Timpson, N.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1341-1348(2010)Wei, Z., et al. J. Biol. Chem. 285(17):12469-12473(2010)Upton, J., et al. N. Z. Med. J. 123 (1308), 123 (2010) :Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.